Journal: Aquaculture Reports

Article Title: The effects of recombinant GnRH with dopamine antagonist on reproduction performance, sex steroid levels, and stress response in female koi carp (Cyprinus carpio)

doi: 10.1016/j.aqrep.2021.101001

Figure Lengend Snippet: Fig. 1. Plasma 17β-estradiol levels in koi carp (Cyprinus carpio) females after injection with 0.9% NaCl with 5 mg/kg domperidone (C); 0.5 ml/kg BW ovaprim (OVP); 10 μg/kg BW rGnRH with 5 mg/kg domperidone (rGn10); 25 μg/kg BW rGnRH with 5 mg/kg domperidone (rGn25); 50 μg/kg BW rGnRH with 5 mg/kg domperidone (rGn50). Mean ± SD; n = 18. Different letters designate significant differences as determined by Tukey’s post-hoc tests.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) commercial kits were applied to measure sex steroid concentrations (ng/ml) of testosterone (T, Cat. No: 3725–300, AccuBind ELISA Microwells, Monobind, Inc. Lake Forest, CA, USA), 17β estradiol (E2, Cat. No: 4925–300, AccuBind ELISA Microwells, Monobind, Inc. Lake Forest, CA, USA), and 17α,20βdihydroxy-4-pregnen-3-one (DHP, Cat. No: CK-E91529, Eastbiopharm, USA).

Techniques: Clinical Proteomics, Injection

Journal: Biomacromolecules

Article Title: Microfluidics-Assisted Fabrication of Gelatin-Silica Core–Shell Microgels for Injectable Tissue Constructs

doi: 10.1021/bm401533y

Figure Lengend Snippet: (a) Fluorescent images of CSP cells on GelMA microgels (left) and GelMA microgels coated with silica hydrogel (right), subjected to oxidative stress. The cells were stained with calcein-AM (green) and ethidium homodimer-1 (red) to visualize live and dead cells, respectively (scale bar: 50 μm). (b) Viability of the CSP cells was quantified as the percentage of the live cells (* p < 0.05).

Article Snippet: After 1 h of incubation, the viability of cells on the microgels was evaluated using LIVE/DEAD Viability Kit (Invitrogen).

Techniques: Staining